Revascularization of decellularized lung scaffolds: principles and progress.

摘要: There is a clear unmet clinical need for novel biotechnology-based therapeutic approaches to lung repair and/or replacement, such as tissue engineering of whole bioengineered lungs. Recent studies have demonstrated the feasibility decellularizing organ by removal all its cellular components, thus leaving behind extracellular matrix complex three-dimensional (3D) biomimetic scaffold. Implantation decellularized scaffolds (DLS), which were recellularized with patient-specific (progenitor) cells, deemed ultimate alternative transplantation. Preclinical that, upon implantation in rodent models, lungs that airway and vascular cells capable gas exchange up 14 days. However, long-term applicability this concept thwarted part failure current reconstruct physiologically functional, quiescent endothelium lining entire tree reseeded scaffolds, inferred from occurrence hemorrhage into compartment thrombosis vasculature vivo. In review, we explore idea successful bioengineering will critically depend on 1) preserving reestablishing integrity subendothelial basement membrane, especially ultrathin respiratory membrane separating airways capillaries, during following decellularization 2) restoring physiological functionality including barrier function quiescence endothelial reseeding compartment. We posit reconstitution pulmonary entirety significantly promote translation next generation