Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form

作者: J. M. Mackenzie , R. A. Coleman , W. R. Briggs , L. H. Pratt

DOI: 10.1073/PNAS.72.3.799

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摘要: Abstract The intracellular localization of phytochrome was seen in dark-grown oat (Avena sativa L., cv. Garry) and rice (Oryza unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. Phytochrome is generally distributed throughout the cytoplasm cells tissue that had not been exposed to prior fixation. Within, at most, 8 min onset saturating red irradiation, phytochrome, now present far-red-absorbing form, becomes associated with discrete regions cell. These do appear be nuclei, plastids, or mitochondria. After phototransformation back red-absorbing form originally present, slowly resumes its general distribution. It possible this represents a physiologically significant binding receptor site

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