Potent inhibition of Hendra virus infection via RNA interference and poly I:C immune activation

摘要: Hendra virus (HeV) is a highly pathogenic zoonotic paramyxovirus that causes fatal disease in wide range of species, including humans. HeV was first described Australia 1994, and has continued to re-emerge with increasing frequency. significant concern human health due its high mortality rate, emergence, absence vaccines limited post exposure therapies. Here we investigate the use RNA interference (RNAi) based therapeutics targeting conjunction TLR3 agonist Poly I:C show they are potent inhibitors infection vitro. We found short interfering RNAs (siRNAs) abundantly expressed N, P M genes caused over 95% reduction titre, protein mRNA. Furthermore, combination siRNA had an additive effect suppressing infection. Our results demonstrate for time RNAi type I interferon stimulation effective replication vitro may provide therapy this lethal, pathogen.