Comparative Genome Analysis of an Extensively Drug-Resistant Isolate of Avian Sequence Type 167 Escherichia coli Strain Sanji with Novel In Silico Serotype O89b:H9.

摘要: ABSTRACT Extensive drug resistance (XDR) is an escalating global problem. Escherichia coli strain Sanji was isolated from outbreak of pheasant colibacillosis in Fujian province, China, 2011. This has XDR properties, exhibiting sensitivity to carbapenems but no other classes known antibiotics. Whole-genome sequencing revealed a total 32 antibiotic genes, many associated with insertion sequence 26 (IS26) elements. These were found on the chromosome and 2 its 6 plasmids, pSJ_255 pSJ_82. The also harbors type secretion system (T2SS), 3 (T3SS), (T6SS), several putative prophages. ST167 strains have previously uncharacterized O-antigen (O89b) that most closely related serotype O89 as determined basis analysis wzm-wzt genes silico serotyping. O89b-antigen gene cluster genomes few pathogenic 617 (ST617) ST10 complex strains. A time-scaled phylogeny inferred comparative single nucleotide variant indicated development these O89b-containing lineages emerged about 30 years ago. Comparative core genome nearly identical recently sequenced E. belonging group. Comparison mobile elements among different each carries distinct set multidrug types indicating there are multiple paths toward emergence coli. IMPORTANCEE. first analyzed novel O89b:H9. sets IS elements, genome, exist achieving XDR. diverse capabilities highlights difficulty preventing or mitigating properties bacteria points importance better understanding shared underlying virulence mechanisms physiology bacteria. Author Video: An author video summary this article available.