Effective siRNA targeting of the 3' untranslated region of the West Nile virus genome.
作者： Karen G. Anthony , Fengwei Bai , Manoj N. Krishnan , Erol Fikrig , Raymond A. Koski
DOI: 10.1016/J.ANTIVIRAL.2008.12.007
关键词:
摘要: West Nile virus (WNV) is an emerging human pathogen for which specific antiviral therapy has not been developed. The therapeutic potential of RNA interference (RNAi) as a sequence-specific inhibitor WNV well demonstrated. Although multiple siRNA targets have identified within the genomic coding region, untranslated regions (UTR), encode cis-acting regulatory elements, remain relatively unknown. In and other flaviviruses, UTRs are located at termini. These form complex secondary structures, pose difficulty when designing effective targets. this study, we report identification in 3' UTR. were selected by predictor algorithms, synthesized short hairpin sequences from plasmid-based expression system. Vero cells stably expressing these had greatly diminished ability to support replication but related dengue virus, demonstrating that siRNAs suppressed viral manner. developed study could function therapeutics genetic tools investigate role UTR pathogenesis.
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David A Stein, Pei-Yong Shi, Nucleic acid-based inhibition of flavivirus infections Frontiers in Bioscience. ,vol. 13, pp. 1385- 1395 ,(2008) , 10.2741/2769
Lewis Markoff, 5′- and 3′-noncoding regions in flavivirus RNA. Advances in Virus Research. ,vol. 59, pp. 177- 228 ,(2003) , 10.1016/S0065-3527(03)59006-6
Brett D. Lindenbach, Charles M. Rice, Molecular biology of flaviviruses. Advances in Virus Research. ,vol. 59, pp. 23- 61 ,(2003) , 10.1016/S0065-3527(03)59002-9
T. Solomon, D. W. Vaughn, Pathogenesis and Clinical Features of Japanese Encephalitis and West Nile Virus Infections Current Topics in Microbiology and Immunology. ,vol. 267, pp. 171- 194 ,(2002) , 10.1007/978-3-642-59403-8_9
Matthew McCown, Michael S Diamond, Andrew Pekosz, The utility of siRNA transcripts produced by RNA polymerase i in down regulating viral gene expression and replication of negative- and positive-strand RNA viruses Virology. ,vol. 313, pp. 514- 524 ,(2003) , 10.1016/S0042-6822(03)00341-6
Konstantin V Pugachev, Farshad Guirakhoo, Dennis W Trent, Thomas P Monath, Traditional and novel approaches to flavivirus vaccines International Journal for Parasitology. ,vol. 33, pp. 567- 582 ,(2003) , 10.1016/S0020-7519(03)00063-8
Bo Zhang, Hongping Dong, David A. Stein, Patrick L. Iversen, Pei-Yong Shi, West Nile virus genome cyclization and RNA replication require two pairs of long-distance RNA interactions. Virology. ,vol. 373, pp. 1- 13 ,(2008) , 10.1016/J.VIROL.2008.01.016
S.P. Ong, B.G.H. Choo, J.J.H. Chu, M.L. Ng, Expression of vector-based small interfering RNA against West Nile virus effectively inhibits virus replication. Antiviral Research. ,vol. 72, pp. 216- 223 ,(2006) , 10.1016/J.ANTIVIRAL.2006.06.005
S.P. Ong, J.J.H. Chu, M.L. Ng, Inhibition of West Nile virus replication in cells stably transfected with vector-based shRNA expression system Virus Research. ,vol. 135, pp. 292- 297 ,(2008) , 10.1016/J.VIRUSRES.2008.04.014
Yongbo Yang, Chengxiang Wu, Jianguo Wu, Vivek R. Nerurkar, Richard Yanagihara, Yuanan Lu, Inhibition of west nile virus replication by retrovirus‐delivered small interfering RNA in human neuroblastoma cells Journal of Medical Virology. ,vol. 80, pp. 930- 936 ,(2008) , 10.1002/JMV.21164