VASP and Profilin-1 Interaction in Cell Migration

摘要: Profilins belong to a family of G-actin binding proteins which are thought facilitate actinpolymerization at the leading edge migrating cells via its polyproline interactions with majoractin nucleating and F-actin elongating proteins. The two major goals this study i) tospatially resolve profilins-1's (the only ubiquitously expressed member profilin family)interaction Ena (enabled)/VASP (vasodilator stimulated phosphoprotein) F-actinelongating protein, ii) determine whether Ena/VASP regulates cell migration through itsinteraction profilin-1. This demonstrates feasibility GFP (green fluorescence protein)-based florescence resonance energy transfer (FRET) identify profilin-1 VASP interaction. Through acceptor photobleaching FRET (fluorescence transfer) inMDA-MB-231 human breast cancer cells, we show that interaction themembrane ruffles near edge. We further overexpression in breastcancer results slower random motility; however VASP-induced suppression cellmotility is partly rescued when VASP:profilin-1 downregulated These data suggestVASP utilizes regulate migration.