Laminin-111 peptide C16 regulates invadopodia activity of malignant cells through β1 integrin, Src and ERK 1/2
作者： Adriane S Siqueira , Monique P Pinto , Mário C Cruz , Basilio Smuczek , Karen SP Cruz
DOI: 10.18632/ONCOTARGET.10062
关键词:
摘要: Laminin peptides influence tumor behavior. In this study, we addressed whether laminin peptide C16 (KAFDITYVRLKF, γ1 chain) would increase invadopodia activity of cells from squamous cell carcinoma (CAL27) and fibrosarcoma (HT1080). We found that stimulates over time in both lines. Rhodamine-conjugated decorates the edge cells, suggesting a possible binding to membrane receptors. Flow cytometry showed increases activated β1 integrin, integrin miRNA-mediated depletion diminishes C16-induced Src ERK 1/2 phosphorylation, inhibition decreases peptide-induced activity. also cortactin phosphorylation Based on our findings, propose regulates probably through signaling pathways.
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