The immunomodulatory effect of sevoflurane in endotoxin-injured alveolar epithelial cells.

摘要: BACKGROUND: Endotoxin-induced lung injury is a useful experimental system for the characterization of immunopathologic mechanisms in acute injury. Although alveolar epithelial cells (AEC) are directly exposed to volatile anesthetics, there limited information about effect anesthetics on these cells. In this study we investigated pretreatment with inhaled anesthetic sevoflurane lipopolysaccharide (LPS)-injured AEC. METHODS: AEC were incubated 1.1 vol % 0.5 h, followed by LPS stimulation 5 h. Expression monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1beta (MIP-1beta), protein-2 (MIP-2), cytokine-induced neutrophil chemoattractant-1 (CINC-1), and intercellular adhesion molecule-1 (ICAM-1) was analyzed. addition, functional tests performed through chemotaxis adherence assays underline biological relevance findings. RESULTS: Exposure resulted 50% downregulation MCP-1 protein sevoflurane-LPS group when compared non-sevoflurane- (P < 0.05). MIP-1beta concentration LPS-stimulated decreased 32% 0.05), MIP-2 29% CINC-1 20% ICAM-1 expression attenuated 36% This inhibition caused substantial changes response neutrophils. 33% less chemotactic activity seen sevoflurane-treated 0.001) as well 47% neutrophils 0.001). CONCLUSIONS: shows that alters LPS-induced response, not only respect pattern mediators, but also regarding consequences accumulation effector such