Fusions to Chloramphenicol Acetyltransferase as a Reporter
作者： Clayton Bullock , Cornelia Gorman
DOI: 10.1016/S0076-6879(00)26056-4
关键词:
摘要: Publisher Summary This chapter presents chloramphenicol acetyltransferase (CAT) as a gene expression tool, the system has been widely employed to characterize transfection techniques for tissues and cell lines, characterizing DNA mutagenesis repair systems, genetic biochemical analyses of transcription factors initiation complexes, characterization signaling pathways, tissue specificity promoters in transgenic animals, evaluating efficacy delivery systems therapy intact animals. The focuses on three types CAT assay methods, thin-layer chromatography (TLC) assay, mixed phase high-performance liquid (HPLC) assay. Parameters that can influence sensitivity reproducibility assays include concentration stability reagents, temperature reaction, reaction time, extraction efficiency products solvents, presence other acetylases volume, type transfected, preparation extracts, amount extracts used reaction.
sci-hub.se 参考文章(32)
Dennis E. Hruby, Elizabeth M. Wilson, Use of fluorescent chloramphenicol derivative as a substrate for chloramphenicol acetyltransferase assays. Methods in Enzymology. ,vol. 216, pp. 369- 376 ,(1992) , 10.1016/0076-6879(92)16034-H
W.V. Shaw, Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods in Enzymology. ,vol. 43, pp. 737- 755 ,(1975) , 10.1016/0076-6879(75)43141-X
W.V. Shaw, The enzymatic acetylation of chloramphenicol by extracts of R factor-resistant Escherichia coli. Journal of Biological Chemistry. ,vol. 242, pp. 687- 693 ,(1967) , 10.1016/S0021-9258(18)96259-9
A L Smith, D H Smith, Improved enzymatic assay of chloramphenicol. Clinical Chemistry. ,vol. 24, pp. 1452- 1457 ,(1978) , 10.1093/CLINCHEM/24.9.1452
Marlyse Knuchel, Daniel P. Bednarik, Nathaniel Chikkala, Francois Villinger, Thomas M. Folks, Aftab A. Ansari, Development of a novel quantitative assay for the measurement of chloramphenicol acetyl transferase (CAT) mRNA Journal of Virological Methods. ,vol. 48, pp. 325- 338 ,(1994) , 10.1016/0166-0934(94)90131-7
Hiroshi Mitani, Jun-Ichiro Komura, Akihiro Shima, The repair of UV-irradiated plasmids transfected into cultured fish cells. Mutation Research-dna Repair. ,vol. 236, pp. 77- 84 ,(1990) , 10.1016/0921-8777(90)90035-4
STEVEN L. YOUNG, ARTHUR E. JACKSON, DAVID PUETT, MICHAEL H. MELNER, Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method. DNA (Mary Ann Liebert, Inc.). ,vol. 4, pp. 469- 475 ,(1985) , 10.1089/DNA.1985.4.469
Merilyn J. Sleigh, A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells. Analytical Biochemistry. ,vol. 156, pp. 251- 256 ,(1986) , 10.1016/0003-2697(86)90180-6
G.H. Smith, P.J. Doherty, R.B. Stead, C.M. Gorman, D.E. Graham, B.H. Howard, Detection of transcription and translation in situ with biotinylated molecular probes in cells transfected with recombinant DNA plasmids Analytical Biochemistry. ,vol. 156, pp. 17- 24 ,(1986) , 10.1016/0003-2697(86)90147-8
Lakshmanan Sankaran, A simple quantitative assay for chloramphenicol acetyltransferase by direct extraction of the labeled product into scintillation cocktail Analytical Biochemistry. ,vol. 200, pp. 180- 186 ,(1992) , 10.1016/0003-2697(92)90296-J