Inhibition of zinc-induced metallothionein mRNA accumulation by gonadotropin-releasing hormone in human hepatocarcinoma cell line HepG2.
作者： Debananda Pati , Hamid R. Habibi , Lashitew Gedamu
DOI: 10.1111/J.1432-1033.1996.0036R.X
关键词:
摘要: Recently we have demonstrated that the human hepatocellular carcinoma-derived cell lines, HepG2 and HuH7, contain gonadotropin-releasing hormone (gonadoliberin) receptors respond to various molecular forms of gonadoliberin in terms suppressed proliferation vitro. This study provides first demonstration inhibits zinc-induced production metallothionein mRNA HuH7 cells. Administration agonist (gonadoliberin-A) inhibited Zn-induced level a time-related dose-related manner. The effect gonadoliberin-A was found be specific, because concomitant treatment with antagonist (gonadoliberin-ANT) blocked inhibition accumulation. Furthermore, gonadoliberin-A-induced Zn-mediated accumulation correlate closely suppresion [3H] thymidine uptake these It is known metal-binding protein plays an important role tumor pathobiology resistance chemotherapeutic drugs. present findings may implications development effective chemotherapy for liver cancer, part, by improving sensitivity cells through suppression peptides.
sci-hub.se 参考文章(23)
C D Klaassen, M P Waalkes, P C Huang, M G Cherian, Y P Liu, D G Longfellow, National Cancer Institute Workshop on the Possible Roles of Metallothionein in Carcinogenesis Cancer Research. ,vol. 53, pp. 922- 925 ,(1993)
N Jahroudi, R Foster, J Price-Haughey, G Beitel, L Gedamu, Cell-type specific and differential regulation of the human metallothionein genes. Correlation with DNA methylation and chromatin structure. Journal of Biological Chemistry. ,vol. 265, pp. 6506- 6511 ,(1990) , 10.1016/S0021-9258(19)39356-1
J. Miller, A.D. McLachlan, A. Klug, Repetitive zinc‐binding domains in the protein transcription factor IIIA from Xenopus oocytes. The EMBO Journal. ,vol. 4, pp. 1609- 1614 ,(1985) , 10.1002/J.1460-2075.1985.TB03825.X
C Sadhu, L Gedamu, Metal-specific posttranscriptional control of human metallothionein genes. Molecular and Cellular Biology. ,vol. 9, pp. 5738- 5741 ,(1989) , 10.1128/MCB.9.12.5738
Tzvia Segal-Abramson, Judith Giat, Joseph Levy, Yoav Sharoni, Guanine nucleotide modulation of high affinity gonadotropin-releasing hormone receptors in rat mammary tumors Molecular and Cellular Endocrinology. ,vol. 85, pp. 109- 116 ,(1992) , 10.1016/0303-7207(92)90130-X
H. Rubin, Inhibition of DNA Synthesis in Animal Cells by Ethylene Diamine Tetraacetate, and Its Reversal by Zinc Proceedings of the National Academy of Sciences of the United States of America. ,vol. 69, pp. 712- 716 ,(1972) , 10.1073/PNAS.69.3.712
Glen K. Andrews, Eileen D. Adamson, Lashitew Gedamu, The ontogeny of expression of murine metallothionein: Comparison with the α-fetoprotein gene Developmental Biology. ,vol. 103, pp. 294- 303 ,(1984) , 10.1016/0012-1606(84)90317-8
Chiharu Tohyama, Junko Sayato Suzuki, Jack Hemelraad, Noriko Nishimura, Hisao Nishimura, Induction of metallothionein and its localization in the nucleus of rat hepatocytes after partial hepatectomy Hepatology. ,vol. 18, pp. 1193- 1201 ,(1993) , 10.1002/HEP.1840180527
Umesh Varshney, Lashitew Gedamu, Human metallothionein MT-I and MT-II processed genes Gene. ,vol. 31, pp. 135- 145 ,(1984) , 10.1016/0378-1119(84)90204-X
D Pati, H R Habibi, Inhibition of human hepatocarcinoma cell proliferation by mammalian and fish gonadotropin-releasing hormones. Endocrinology. ,vol. 136, pp. 75- 84 ,(1995) , 10.1210/ENDO.136.1.7828560