Three Distinct Patterns of Histone H3Y41 Phosphorylation Mark Active Genes

摘要: The JAK2 tyrosine kinase is a critical mediator of cytokine-induced signaling. It plays role in the nucleus, where it regulates transcription by phosphorylating histone H3 at 41 (H3Y41ph). We used chromatin immunoprecipitation coupled to massively parallel DNA sequencing (ChIP-seq) define genome-wide pattern H3Y41ph human erythroid leukemia cells. Our results indicate that located three distinct sites: (1) at a subset active promoters, overlaps with H3K4me3, (2) distal cis-regulatory elements, coincides binding STAT5, and (3) throughout transcribed regions active, tissue-specific hematopoietic genes. Together, these data extend our understanding this conserved essential signaling pathway provide insight into mechanisms which extracellular stimuli may lead coordinated regulation transcription.