Microscopy of the response of rodent dentin to injected fluoride

摘要: Rats were injected subcutaneously with 50 mg/kg body weight of sodium fluoride. The hypermineralized and hypomineralized components the response in incisor dentin identified ground sections using contact microradiography analyzed microscopically for matrix alterations. In demineralized component stained weakly acid polysaccharide (alcian blue, Hale's iron method), was less dense (interference microscopy) more birefringent than normal matrix. intensely electron microscope, inorganic crystals numerous larger normal, while those but diameters. These observations indicate that results from an inhibition polysaccharide-collagen interaction, resulting a paucity apatite nucleation sites soluble organic increased period time aggregation formation therefore presence nearly density indicates mature rodent is completely mineralized bone osteons.