Cx43 Present at the Leading Edge Membrane Governs Promigratory Effects of Osteoblast-Conditioned Medium on Human Prostate Cancer Cells in the Context of Bone Metastasis.

摘要: Among the different interacting molecules implicated in bone metastases, connexin43 (Cx43) may increase sensitivity of prostate cancer (PCa) cells to microenvironment, as suggested by our silico and human tissue samples analyses that revealed increased level Cx43 expression with PCa progression a specific secondary sites. The goal present study was understand how influences aggressiveness microenvironment. By means Cx43-overexpressing cell lines, we Cx43-dependent promigratory effect osteoblastic conditioned media (ObCM). This on directional migration relied presence at plasma membrane not gap junctional intercellular communication hemichannel functions. ObCM stimulation induced Rac1 activation interaction cortactin protrusions migrating cells. Finally, transfecting two truncated forms LNCaP cells, determined carboxy terminal (CT) part is crucial for responsiveness ObCM. Our demonstrates its localization modulate phenotypic response microenvironment CT domain plays pivotal role.