Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells.
作者： Chengle Zhuang , Jianfa Li , Yuchen Liu , Mingwei Chen , Jiancheng Yuan
关键词:
摘要: Recent studies show that long non-coding RNAs (lncRNAs) may be significant functional regulators in tumor development, including bladder cancer. Here, we found PVT1 was upregulated cancer tissues and cells. Further experiments revealed promoted cell proliferation suppressed apoptosis. Furthermore also used the emerging technology, synthetic biology, to create tetracycline-inducible small hairpin RNA (shRNA) vectors which silenced a dosage-dependent manner inhibit progression of In conclusion, data suggest could an oncogene therapeutic target Synthetic "tetracycline-on" switch system can quantitatively control expression response different concentration doxycycline suppress
impactjournals.com参考文章(30)
Enling Liu, Yuxiu Zhou, Zheng Liu, Carboplatin-docetaxel-induced activity against ovarian cancer is dependent on up-regulated lncRNA PVT1. International Journal of Clinical and Experimental Pathology. ,vol. 8, pp. 3803- 3810 ,(2015)
Feng Wang, Hou-Qun Ying, Bang-Shun He, Yu-Qin Pan, Qi-Wen Deng, Hui-Ling Sun, Jie Chen, Xian Liu, Shu-Kui Wang, Upregulated lncRNA-UCA1 contributes to progression of hepatocellular carcinoma through inhibition of miR-216b and activation of FGFR1/ERK signaling pathway. Oncotarget. ,vol. 6, pp. 7899- 7917 ,(2015) , 10.18632/ONCOTARGET.3219
Hao Bo, Zhaojian Gong, Wenling Zhang, Xiayu Li, Yong Zeng, Qianjin Liao, Pan Chen, Lei Shi, Yu Lian, Yizhou Jing, Ke Tang, Zheng Li, Yanhong Zhou, Ming Zhou, Bo Xiang, Xiaoling Li, Jianbo Yang, Wei Xiong, Guiyuan Li, Zhaoyang Zeng, Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget. ,vol. 6, pp. 20404- 20418 ,(2015) , 10.18632/ONCOTARGET.4057
Ho Kyung Seo, Kyung-Ohk Ahn, Nae-Rae Jung, Ji-Sun Shin, Weon Seo Park, Kang Hyun Lee, Sang-Jin Lee, Kyung-Chae Jeong, Antitumor activity of the c-Myc inhibitor KSI-3716 in gemcitabine-resistant bladder cancer Oncotarget. ,vol. 5, pp. 326- 337 ,(2014) , 10.18632/ONCOTARGET.1545
S. Goverdhana, M. Puntel, W. Xiong, J.M. Zirger, C. Barcia, J.F. Curtin, E.B. Soffer, S. Mondkar, G.D. King, J. Hu, S.A. Sciascia, M. Candolfi, D.S. Greengold, P.R. Lowenstein, M.G. Castro, Regulatable Gene Expression Systems for Gene Therapy Applications: Progress and Future Challenges Molecular Therapy. ,vol. 12, pp. 189- 211 ,(2005) , 10.1016/J.YMTHE.2005.03.022
Pedro J. Batista, Howard Y. Chang, Long Noncoding RNAs: Cellular Address Codes in Development and Disease Cell. ,vol. 152, pp. 1298- 1307 ,(2013) , 10.1016/J.CELL.2013.02.012
Yongmao Yu, Alka Arora, Weixian Min, Chaim M. Roifman, Eyal Grunebaum, EdU incorporation is an alternative non-radioactive assay to [3H]thymidine uptake for in vitro measurement of mice T-cell proliferations Journal of Immunological Methods. ,vol. 350, pp. 29- 35 ,(2009) , 10.1016/J.JIM.2009.07.008
Yuen-Yi Tseng, Branden S. Moriarity, Wuming Gong, Ryutaro Akiyama, Ashutosh Tiwari, Hiroko Kawakami, Peter Ronning, Brian Reuland, Kacey Guenther, Thomas C. Beadnell, Jaclyn Essig, George M. Otto, M. Gerard O’Sullivan, David A. Largaespada, Kathryn L. Schwertfeger, York Marahrens, Yasuhiko Kawakami, Anindya Bagchi, PVT1 dependence in cancer with MYC copy-number increase Nature. ,vol. 512, pp. 82- 86 ,(2014) , 10.1038/NATURE13311
Cheng-Le Zhuang, Xing Fu, Li Liu, Yu-Chen Liu, Wei-Ren Huang, Zhi-Ming Cai, Synthetic miRNA sponges driven by mutant hTERT promoter selectively inhibit the progression of bladder cancer. Tumor Biology. ,vol. 36, pp. 5157- 5163 ,(2015) , 10.1007/S13277-015-3169-9
Yuchen Liu, Yayue Zeng, Li Liu, Chengle Zhuang, Xing Fu, Weiren Huang, Zhiming Cai, Synthesizing AND gate genetic circuits based on CRISPR-Cas9 for identification of bladder cancer cells Nature Communications. ,vol. 5, pp. 5393- ,(2014) , 10.1038/NCOMMS6393