Novel 1,2,3-triazolium-based dicationic amphiphiles synthesized using click-chemistry approach for efficient plasmid delivery.

摘要: Herein, we report the synthesis, characterization and evaluation of transfection efficiencies a series dicationic amphiphiles designed to construct quaternary ammonium ion-based cationic lipids varying in chain length hydrophobic back bone connected individually through head group 1,2,3-triazolium cation consisting 2-hydroxy ethyl as substitution. Accordingly, three were synthesized by “click chemistry” approach formulated bilayered vesicles using DOPE co-lipid. The efficacies these novel lipid formulations measured correlated with results obtained from various physicochemical techniques. Importantly, observed gradient activity profile, where potential increased decreasing bone, highlights synergistic interplay alkyl coordination charge delocalization modulating potency 1,2,3-triazolium-based lipids.