Immunophenotyping and DNA content analysis of acetone-fixed cells.
作者： Debora Mancaniello , Maurizio Carbonari
DOI: 10.1002/0471142956.CY0926S46
关键词:
摘要: The flow acetone staining technique (FAST) allows one to concurrently study physical cell features revealed by light-scatter analysis, surface/nuclear phenotypes, and cellular DNA content. Thus, diverse subpopulations of proliferating cells can be identified in heterogeneous populations their immunophenotype cycle status, ploidy assessed. Acetone, a coagulant (precipitating) fixative that also has the ability permeabilize membranes, is widely used static cytometry, but rarely cytometry because its undesirable effects, namely causing shrinkage. Nevertheless, when employed under proper temperature conditions (∼8°C), it preserves well, compatible with stoichiometric accurate measurement Due these virtues FAST, method provides useful approaches for biology hematology/oncology studies. Curr. Protocol. Cytom. 46:9.26.1-9.26.11. © 2008 John Wiley & Sons, Inc. Keywords: acetone; multiparametric cytometry; DNA content; conservative fixation; proliferating
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