Purification of rabbit skeletal muscle protein kinase regulatory subunit using cyclic adenosine-3':5'-monophosphate affinity chromatography.
作者： William L. Dills , Joseph A. Beavo , Peter J. Bechtel , Edwin G. Krebs
DOI: 10.1016/S0006-291X(75)80406-2
关键词:
摘要: Summary A method for the preparation of homogeneous protein kinase regulatory subunit has been developed using adenosine-3′,5′-monophosphate (cAMP) affinity chromatography. N 6 -(2-Aminoethyl)-cAMP was synthesized and covalently linked to CNBr-activated Sepharose 4B. When a crude rabbit skeletal muscle applied ge1, cAMP independent activity recovered in effluent. The bound not removed with 2 M NaC1 but could be eluted 30 mM cAMP. This identical prepared by other methods as judged electrophoretic migration presence sodium dodecylsulfate, binding, inhibition catalytic absence
elsevier.com
sci-hub.se 参考文章(15)
Donal A. Walsh, Edwin G. Krebs, 16 Protein Kinases The Enzymes. ,vol. 8, pp. 555- 581 ,(1973) , 10.1016/S1874-6047(08)60076-1
IM Glynn, JB Chappell, A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity Biochemical Journal. ,vol. 90, pp. 147- 149 ,(1964) , 10.1042/BJ0900147
Donal A. Walsh, John P. Perkins, Charles O. Brostrom, Ester S. Ho, Edwin G. Krebs, Catalysis of the Phosphorylase Kinase Activation Reaction Journal of Biological Chemistry. ,vol. 246, pp. 1968- 1976 ,(1971) , 10.1016/S0021-9258(19)77176-2
Charles S. Rubin, Jack Erlichman, Ora M. Rosen, Molecular Forms and Subunit Composition of a Cyclic Adenosine 3',5'-Monophosphate-dependent Protein Kinase Purified from Bovine Heart Muscle Journal of Biological Chemistry. ,vol. 247, pp. 36- 44 ,(1972) , 10.1016/S0021-9258(19)45755-4
KAZUO SATAKE, TSUNEO OKUYAMA, MOCHIHIKO OHASHI, TOMOTAKA SHINODA, The spectrophotometric determination of amine, amino acid and peptide with 2,4,6-trinitrobenzene 1-sulfonic acid. Journal of Biochemistry. ,vol. 47, pp. 654- 660 ,(1960) , 10.1093/OXFORDJOURNALS.JBCHEM.A127107
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Erwin M. Reimann, Donal A. Walsh, Edwin G. Krebs, Purification and properties of rabbit skeletal muscle adenosine 3',5'-monophosphate-dependent protein kinases. Journal of Biological Chemistry. ,vol. 246, pp. 1986- 1995 ,(1971) , 10.1016/S0021-9258(19)77178-6
Bruce N. Ames, ASSAY OF INORGANIC PHOSPHATE, TOTAL PHOSPHATE AND PHOSPHATASE Methods in Enzymology. ,vol. 8, pp. 115- 118 ,(1966) , 10.1016/0076-6879(66)08014-5
Judith Ramseyer, Harvey R. Kaslow, Gordon N. Gill, Purification of the cAMP receptor protein by affinity chromatography Biochemical and Biophysical Research Communications. ,vol. 59, pp. 813- 821 ,(1974) , 10.1016/S0006-291X(74)80052-5
Gordon N. Gill, Leonard D. Garren, A cyclic-3′,5′-adenosine monophosphate dependent protein kinase from the adrenal cortex: Comparison with a cyclic AMP binding protein Biochemical and Biophysical Research Communications. ,vol. 39, pp. 335- 343 ,(1970) , 10.1016/0006-291X(70)90581-4