The etr1-2 mutation in Arabidopsis thaliana affects the abscisic acid, auxin, cytokinin and gibberellin metabolic pathways during maintenance of seed dormancy, moist-chilling and germination

摘要: In Arabidopsis thaliana, the etr1-2 mutation confers dominant ethylene insensitivity and results in a greater proportion of mature seeds that exhibit dormancy compared with wild-type. We investigated impact on other plant hormones by analyzing profiles four classes their metabolites HPLC-ESI/MS/MS wild-type plants. Hormone were analyzed imbibed immediately under germination conditions, subjected to 7-day moist-chilling (stratification) period, during germination/early post-germinative growth. Higher than levels abscisic acid (ABA) appeared contribute, at least part, incidence etr1-2. The lower glucose ester (ABA-GE) conditions (with without treatments) suggest reduced metabolism ABA ABA-GE likely contributed accumulation mutant. mutant exhibited generally higher auxin large build-up indole-3-aspartate when placed following moist-chilling. manifested increased cytokinin glucosides through zeatin-O-glucosylation (Z-O-Glu). resulting increase Z-O-Glu was largest most consistent change associated ETR1 gene mutation. There more gibberellins (GA) concentrations Our signaling modulates all hormone pathways seeds. Additionally, seed requirement for GA promote absence functional pathway.