Phytate addition to soil induces changes in the abundance and expression of Bacillus ß-propeller phytase genes in the rhizosphere

作者: Milko A. Jorquera , Nicolás Saavedra , Fumito Maruyama , Alan E. Richardson , David E. Crowley

DOI: 10.1111/J.1574-6941.2012.01480.X

关键词:

摘要: Phytate-mineralizing rhizobacteria (PMR) perform an essential function for the mineralization of organic phosphorus but little is known about their ecology in soils and rhizosphere. In this study, PCR-based methods were developed detection quantification Bacillus β-propeller phytase (BPP) gene. Experiments conducted to monitor presence persistence a phytate-mineralizing strain, sp. MQH19, after inoculation soil microcosms within The occurrence BPP gene natural pasture from Chilean Andisols was also examined. results showed that readily detected sterile nonsterile microcosms, quantitative PCR (qPCR) could be used changes abundance over time. Our show addition phytate induced expression rhizosphere ryegrass all sampled. This study shows genes demonstrates cosmopolitan Andisols.

参考文章(26)
Edward J Mullaney, Abul HJ Ullah, B Turner, A Richardson, E Mullaney, Phytases: attributes, catalytic mechanisms and applications. Inositol phosphates: linking agriculture and the environment. pp. 97- 110 ,(2007) , 10.1079/9781845931520.0097
Miroslav Radojevic, Vladimir N Bashkin, Practical Environmental Analysis ,(1999)
Jane E Hill, Alan E Richardson, BL Turner, Isolation and assessment of microorganisms that utilize phytate. CABI. pp. 61- 77 ,(2007) , 10.1079/9781845931520.0061
Milko A. Jorquera, David E. Crowley, Petra Marschner, Ralf Greiner, María Teresa Fernández, Daniela Romero, Daniel Menezes-Blackburn, María De La Luz Mora, Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils FEMS Microbiology Ecology. ,vol. 75, pp. 163- 172 ,(2011) , 10.1111/J.1574-6941.2010.00995.X
Janne Kerovuo, Marko Lauraeus, Päivi Nurminen, Nisse Kalkkinen, Juha Apajalahti, Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis. Applied and Environmental Microbiology. ,vol. 64, pp. 2079- 2085 ,(1998) , 10.1128/AEM.64.6.2079-2085.1998
Milko A. Jorquera, Baby Shaharoona, Sajid M. Nadeem, María de la Luz Mora, David E. Crowley, Plant growth-promoting rhizobacteria associated with ancient clones of creosote bush (Larrea tridentata). Microbial Ecology. ,vol. 64, pp. 1008- 1017 ,(2012) , 10.1007/S00248-012-0071-5
F. Borie, R. Rubio, TOTAL AND ORGANIC PHOSPHORUS IN CHILEAN VOLCANIC SOILS Gayana Botanica. ,vol. 60, pp. 69- 73 ,(2003) , 10.4067/S0717-66432003000100011
Milko A. Jorquera, Marcela Hernández, Oscar Martínez, Petra Marschner, María de la Luz Mora, Detection of aluminium tolerance plasmids and microbial diversity in the rhizosphere of plants grown in acidic volcanic soil European Journal of Soil Biology. ,vol. 46, pp. 255- 263 ,(2010) , 10.1016/J.EJSOBI.2010.03.005
Peter M. Groffman, Melany C. Fisk, Phosphate additions have no effect on microbial biomass and activity in a northern hardwood forest Soil Biology & Biochemistry. ,vol. 43, pp. 2441- 2449 ,(2011) , 10.1016/J.SOILBIO.2011.08.011
David Kysela, Menachem Elimelech, Jane E. Hill, Isolation and assessment of phytate-hydrolysing bacteria from the DelMarVa Peninsula. Environmental Microbiology. ,vol. 9, pp. 3100- 3107 ,(2007) , 10.1111/J.1462-2920.2007.01420.X