Multiphoton-Excitation Fluorescence Microscopy and Membranes
关键词:
摘要: In the last few years fluorescence microscopy has become a very useful technique to study lateral structure of membranes. Several experimental strategies using techniques (epifluorescence, confocal and two-photon excitation) have appeared in literature employing GUVs as model systems. The advantages disadvantages different approaches, including use fluorescent probes, were discussed this chapter. particular, polarity-sensitive probe LAURDAN marker under two-photon-excitation microscope not only artificial lipid mixtures but also natural extracts cell membranes was addressed. visual information obtained with these is extremely correlate compositionally simple that observed complex such biological
springer.com
harvard.edu
springerlink.com参考文章(76)
Luis A. Bagatolli, Enrico Gratton, Direct Observation of Lipid Domains in Free-Standing Bilayers Using Two-Photon Excitation Fluorescence Microscopy Journal of Fluorescence. ,vol. 11, pp. 141- 160 ,(2001) , 10.1023/A:1012228631693
L. A. Bagatolli, D. D. Binns, D. M. Jameson, J. P. Albanesi, Activation of dynamin II by POPC in giant unilamellar vesicles: a two-photon fluorescence microscopy study. Journal of Protein Chemistry. ,vol. 21, pp. 383- 391 ,(2002) , 10.1023/A:1021126415320
Brian Herman, X. F. Wang, Fluorescence imaging spectroscopy and microscopy J. Wiley. ,(1996)
Yaakov Bar-Shalom, Applications and advances Artech House. ,(1992)
Tiziana Parasassi, Ewa K. Krasnowska, Luis Bagatolli, Enrico Gratton, Laurdan and Prodan as Polarity-Sensitive Fluorescent Membrane Probes Journal of Fluorescence. ,vol. 8, pp. 365- 373 ,(1998) , 10.1023/A:1020528716621
Luis A. Bagatolli, Susana A. Sanchez, Theodore Hazlett, Enrico Gratton, Giant vesicles, Laurdan, and two-photon fluorescence microscopy: evidence of lipid lateral separation in bilayers. Methods in Enzymology. ,vol. 360, pp. 481- 500 ,(2003) , 10.1016/S0076-6879(03)60124-2
Peter T. C. So, Chen Y. Dong, Barry R. Masters, Keith M. Berland, Two-Photon Excitation Fluorescence Microscopy Annual Review of Biomedical Engineering. ,vol. 2, pp. 399- 429 ,(2000) , 10.1146/ANNUREV.BIOENG.2.1.399
M. Caffrey, F.S. Hing, A temperature gradient method for lipid phase diagram construction using time-resolved x-ray diffraction Biophysical Journal. ,vol. 51, pp. 37- 46 ,(1987) , 10.1016/S0006-3495(87)83309-X
Kaushik Nag, Jin-Si Pao, Robert R. Harbottle, Fred Possmayer, Nils O. Petersen, Luis A. Bagatolli, Segregation of Saturated Chain Lipids in Pulmonary Surfactant Filmsand Bilayers Biophysical Journal. ,vol. 82, pp. 2041- 2051 ,(2002) , 10.1016/S0006-3495(02)75552-5
Philippe Girard, Jacques Pécréaux, Guillaume Lenoir, Pierre Falson, Jean-Louis Rigaud, Patricia Bassereau, A New Method for the Reconstitution of Membrane Proteins into Giant Unilamellar Vesicles Biophysical Journal. ,vol. 87, pp. 419- 429 ,(2004) , 10.1529/BIOPHYSJ.104.040360