Structure of helical RecA-DNA complexes: II. Local conformational changes visualized in bundles of RecA-ATPγS filaments☆☆☆
作者： Edward H. Egelman , Andrzej Stasiak
DOI: 10.1016/0022-2836(88)90245-8
关键词:
摘要: Complexes of RecA-DNA filaments, formed in the presence a non-hydrolyzable ATP analog, gamma S, aggregate together into regular bundles Mg2+. Electron micrographs several different forms RecA-double-stranded DNA have been analyzed: six supercoiled filaments at two concentrations Mg2+, and three single concentration The are all characterized by left-handed supercoiling component arising from non-integral number RecA subunits per turn helix these aggregates, about 6.15 units/turn. When single-stranded is used instead double-stranded DNA, aggregates composed many formed. These do not supercoil, consistent with symmetry close to 6.0 structures provided strong confirmation analysis isolated filaments. Since protomers within environments, they direct view conformations that may adopt same filament as result nonequivalent contacts. conformational changes we visualized quite large, apparent movements mass over distances greater than 2 nm. RecA-mediated strand exchange reaction highly dynamic process, which involves both unwinding stretching addition physical movement strands. It likely, therefore, seen represent states during its enzymatic activity.
elsevier.com
sci-hub.se 参考文章(17)
Edward H. Egelman, Andrzej Stasiak, Structure of helical RecA-DNA complexes. Complexes formed in the presence of ATP-gamma-S or ATP. Journal of Molecular Biology. ,vol. 191, pp. 677- 697 ,(1988) , 10.1016/0022-2836(86)90453-5
M. Takahashi, C. Strazielle, J. Pouyet, M. Daune, Co-operativity value of DNA-RecA protein interaction Journal of Molecular Biology. ,vol. 189, pp. 711- 714 ,(1986) , 10.1016/0022-2836(86)90501-2
S M Honigberg, D K Gonda, J Flory, C M Radding, The pairing activity of stable nucleoprotein filaments made from recA protein, single-stranded DNA, and adenosine 5'-(gamma-thio)triphosphate Journal of Biological Chemistry. ,vol. 260, pp. 11845- 11851 ,(1985) , 10.1016/S0021-9258(17)39108-1
A. Stasiak, E. Di Capua, Th. Koller, Elongation of duplex DNA by recA protein Journal of Molecular Biology. ,vol. 151, pp. 557- 564 ,(1981) , 10.1016/0022-2836(81)90010-3
D.F. Dombroski, D.G. Scraba, R.D. Bradley, A.R. Morgan, Studies of the interaction of RecA protein with DNA Nucleic Acids Research. ,vol. 11, pp. 7487- 7504 ,(1983) , 10.1093/NAR/11.21.7487
Charles M. Radding, Recombination activities of E. coli RecA protein Cell. ,vol. 25, pp. 3- 4 ,(1981) , 10.1016/0092-8674(81)90224-5
Kathi Dunn, Susan Chrysogelos, Jack Griffith, Electron microscopic visualization of RecA-DNA filaments: Evidence for a cyclic extension of duplex DNA Cell. ,vol. 28, pp. 757- 765 ,(1982) , 10.1016/0092-8674(82)90055-1
D.J. DeRosier, P.B. Moore, Reconstruction of three-dimensional images from electron micrographs of structures with helical symmetry☆ Journal of Molecular Biology. ,vol. 52, pp. 355- 369 ,(1970) , 10.1016/0022-2836(70)90036-7
A. Stasiak, E.H. Egelman, Structure and Dynamics of recA Protein-DNA Complexes as Determined by Image Analysis of Electron Micrographs Biophysical Journal. ,vol. 49, pp. 5- 7 ,(1986) , 10.1016/S0006-3495(86)83569-X
Susan M. Cotterill, Alan R. Fersht, recA Filaments in solution Biochemistry. ,vol. 22, pp. 3525- 3531 ,(1983) , 10.1021/BI00283A034