D-glyceraldehyde-3-phosphate dehydrogenase from HeLa cells--1. Purification and properties of the enzyme.
作者： Momoyo Nakano , Shigehiro Funayama , Maria Benigna M. de Oliveira , Sung Lie Bruel , Elisa Maines Gomes
DOI: 10.1016/0305-0491(92)90095-9
关键词:
摘要: Abstract 1. d -GDPH from HeLa cells was isolated and purified. 2. Some basic kinetic constants are reported. 3. Sodium dodecyl polyacrylamide gel electrophoresis gave a single band with molecular weight of approximately 36 K. 4. ATP NADH inhibit competitively enzyme activity. 5. Comparative catalytic properties GPDH normal tumor were effectuated.
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