Application of 1,1′-Carbonyldiimidazone-activated agarose for the purification of proteins
作者： Milton T. W. Hearn , Geoffry S. Bethell , John S. Ayers , William S. Hancock
DOI: 10.1016/S0021-9673(00)85622-8
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摘要: Abstract The use of 1,1′-carbonyldimizale-activated agarose for biospecific affinity chromatography is described. Activation agrose with this carbonylating reagent gives a matrix devoid additional charged groups. Conditions the coupling rannge ligands and leashes have been evaluated. efficient purification bovine trypsin, human thyroglobulin sheep thyroid membrane glycoproteins demonstrates suitabliliy new activated chromatography.
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