Catalase Immobilization on Electrospun Nanofibers: Effects of Porphyrin Pendants and Carbon Nanotubes
作者： Ling-Shu Wan , Bei-Bei Ke , Jian Wu , Zhi-Kang Xu
DOI: 10.1021/JP070983N
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摘要: Carbon nanotube (CNT) has been generally recognized as an electron acceptor, whereas porphyrin can act donor. In this work, acrylonitrile-based copolymers bearing pendants were therefore blended with or without CNTs and then electrospun into nanofibers on which a redox enzyme, catalase, was covalently immobilized. The uniform diameters are around 180 nm. Field emission scanning microscopy transmission confirmed the morphologies of distribution CNTs. Rougher surfaces well obvious protruded parts induced by blending observed from studied nanofibers. Catalase immobilized onto nanofiber surface through activation carboxyl groups N-(3-dimethylaminopropyl)-N‘-ethylcarbodiimide hydrochloride N-hydroxysuccinimide (EDC/NHS). Results indicate that both introduction obviously improve activity stabilities catalases...
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