The Role of the Pinhole in Confocal Imaging Systems

摘要: Confocal scanning microscopes are particularly attractive by virtue of their enhanced lateral resolution, purely coherent image formation and optical sectioning (Wilson Sheppard, 1984). It is probably the latter property which most useful as it gives rise to ability a thick specimen in three-dimensions. This possible because system images information only from thin region neighbourhood focal plane. permits us store many slices computer give three dimensional data set describes object. There now sophisticated software systems available display this various ways. Obvious examples include extended focus technique Hamilton, 1982) we merely add up (integrate) depths provide an greatly depth field. We may also produce where object height coded brightness or combine whole isometric view object, Figure 1. ;also use false color label features interest or, simple processing, obtain stereoscopic pairs (van der Voort, 1985). Other forms discussed Chapter 10.