Prostaglandin Endoperoxides. Novel Transformations of Arachidonic Acid in Human Platelets

摘要: Abstract Arachidonic acid incubated with human platelets was converted into three compounds, 12L-hydroxy-5,8,10,14-eicosatetraenoic acid, 12L-hydroxy-5,8,10-heptadecatrienoic and the hemiacetal derivative of 8-(1-hydroxy-3-oxopropyl)-9,12L-dihydroxy-5,10-heptadecadienoic acid. The formation two latter compounds from arachidonic proceeded by pathways involving enzyme, fatty cyclo-oxygenase, in initial step prostaglandin endoperoxide, PGG2, as an intermediate. first mentioned compound formed 12L-hydroperoxy-5,8,10,14-eicosatetraenoic which turn action a novel lipoxygenase. Aspirin indomethacin inhibited cyclo-oxygenase but not lipoxygenase, whereas 5,8,11,14-eicosatetraynoic both enzymes. almost exclusive transformation endoperoxide structure non-prostaglandin derivatives supports hypothesis that endoperoxides can participate directly way classical prostaglandins regulation cell functions. observed transformations also explain aggregating effect this