Studies of IgE-dependent Histamine Releasing Factors: Heterogeneity of IgE

摘要: Nasal lavage fluids from unstimulated individuals contain a histamine-releasing factor (HRF) similar to those which we have previously described macrophages, platelets, and blister obtained during the late cutaneous reaction. The nasal HRF was partially purified by ion-exchange chromatography gel filtration. Although some m.w. heterogeneity observed, majority of eluted at an apparent range 15,000 30,000. This induced histamine release basophils certain individuals. Histamine occurred via mechanism is IgE-dependent in that: desensitized exposure anti-IgE absence calcium no longer respond HRF, desensitization with reduces responsiveness anti-IgE; removal IgE basophil surface using lactic acid renders cells unresponsive HRF. We further defined this dependence shown that reason only selected donors due unrecognized, functional IgE. Thus, passive sensitization sera responders restored acid-stripped conferred nonresponder naturally unoccupied receptors. Sera nonresponders failed do even though numbers molecules were put onto each case. property responder because both heating 56 degrees C for 2 hr passage over anti-IgE-Sepharose (which removes greater than 90% IgE) markedly reduced ability induce responsiveness, excess either myeloma or penicillin-specific antibody competitively inhibited conclude induces specific, fashion but appropriate type Because are recruited into nose late-phase reaction, suggest may these other mediators could contribute symptomatology