Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property
作者： Chang-Hao Cui , Da Jung Kim , Suk-Chae Jung , Sun-Chang Kim , Wan-Taek Im
DOI: 10.3390/MOLECULES22050844
关键词:
摘要: Minor ginsenosides, such as compound K, Rg3(S), which can be produced by deglycosylation of ginsenosides Rb1, showed strong anti-cancer effects. However, the anticancer effects gypenoside LXXV, is one deglycosylated shapes ginsenoside still unknown due to rarity its content in plants. Here, we cloned and characterized a novel ginsenoside-transforming β-glucosidase (BglG167b) derived from Microbacterium sp. Gsoil 167 efficiently hydrolyze XVII into applied it production LXXV at gram-scale with high specificity. In addition, activity was investigated against three cancer cell lines (HeLa, B16, MDA-MB231) vitro. Gypenoside significantly reduced viability, displaying an enhanced effect compared Rb1. Taken together, this enzymatic method would useful preparation for functional food pharmaceutical industries.
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