Control of mammalian sex ratio by sexing sperm**Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract no. W-7405-ENG-48.

作者: Barton L. Gledhill

DOI: 10.1016/S0015-0282(16)47410-8

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摘要: Preselection of sex is discussed with emphasis on methods which have claimed success in separating X- and Y-chromosome-bearing sperm. Much the recent experimental work human X Y sperm judges enrichment solely by staining for a quinacrine dye, causes bright fluorescence long arm chromosome. This method questioned because endpoint may be producing spurious results. Flow sorting believed to first verified separation mammalian sperm, but were nonviable. cytometry can used quickly determine other techniques. Bulk separation, as contrasted based determination individual characteristics, 80% seems reasonable future goal.

参考文章(11)
Satoru Kaneko, Jun Yamaguchi, Toshifumi Kobayashi, Rihachi Iizuka, Separation of human X- and Y-bearing sperm using Percoll density gradient centrifugation Fertility and Sterility. ,vol. 40, pp. 661- 665 ,(1983) , 10.1016/S0015-0282(16)47427-3
George E. Seidel, Rupert P. Amann, Prospects for sexing mammalian sperm Colorado Associated University Press. ,(1982)
R. H. Martin, W. Balkan, Kathy Burns, A. W. Rademaker, C. C. Lin, N. L. Rudd, The chromosome constitution of 1000 human spermatozoa. Human Genetics. ,vol. 63, pp. 305- 309 ,(1983) , 10.1007/BF00274750
R. J. ERICSSON, C. N. LANGEVIN, M. NISHINO, Isolation of fractions rich in human Y sperm. Nature. ,vol. 246, pp. 421- 424 ,(1973) , 10.1038/246421A0
D Pinkel, B. Gledhill, S Lake, D Stephenson, M. Van Dilla, Sex preselection in mammals? Separation of sperm bearing Y and "O" chromosomes in the vole Microtus oregoni Science. ,vol. 218, pp. 904- 906 ,(1982) , 10.1126/SCIENCE.6753153
H. Fujimoto, R.P. Erickson, Functional assays for mRNA detect many new messages after male meiosis in mice Biochemical and Biophysical Research Communications. ,vol. 108, pp. 1369- 1375 ,(1982) , 10.1016/S0006-291X(82)80057-0
R. A. BEATTY, THE GENETICS OF THE MAMMALIAN GAMETE Biological Reviews. ,vol. 45, pp. 73- 117 ,(1970) , 10.1111/J.1469-185X.1970.TB01076.X
D. L. Garner, B. L. Gledhill, D. Pinkel, S. Lake, D. Stephenson, M. A. Van Dilla, L. A. Johnson, Quantification of the X- and Y-chromosome-bearing spermatozoa of domestic animals by flow cytometry. Biology of Reproduction. ,vol. 28, pp. 312- 321 ,(1983) , 10.1095/BIOLREPROD28.2.312
W. E. Beal, Lydia M. White, Duane L. Garner, Sex ratio after insemination of bovine spermatozoa isolated using a bovine serum albumin gradient. Journal of Animal Science. ,vol. 58, pp. 1432- 1436 ,(1984) , 10.2527/JAS1984.5861432X
B. C. Bhattacharya, Guide to pre-arranging the sex of offspring. Guide to pre-arranging the sex of offspring.. ,(1977)