A multi-generation analysis of the stability of transgenic virus resistance in doubled-haploid tobacco lines.
作者： Yan Sheng Liu , Jennifer S. Levin , Jeffrey S. Murray , Earl A. Wernsman , Arthur K. Weissinger
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摘要: Plants can be genetically engineered for virus resistance by transformation with a viral gene. We transformed tobacco the tomato spotted wilt (TSWV) nucleocapsid gene from Hawaiian L isolate in order to obtain TSWV resistant breeding lines. Doubled-haploid lines were produced primary transgenic plants that selected virus. Several of these showed very high levels and symptomless after inoculation TSWV. The accumulation only low full-length transgene RNA protein observed is consistent an RNA-mediated mechanism resistance. highly also found several other isolates TSWV, while moderately often susceptible isolates. advanced over generations self-pollination. Although fully homozygous, lost later generations, indicating was unstable. Selection unstable did not prevent occurrence progeny subsequent generations. Therefore, testing required determine stability when crops
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Pravda Stoeva, Margarita Yankulova, Velichka Nikolaeva, Rositza Bachvarova, Lidia Ivanova, Edgar Maiss, Guenter Adam, Vladimir Vulkov, Spas Guelemerov, Atanas Atanassov, Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene: greenhouse and field tests Molecular Breeding. ,vol. 4, pp. 155- 164 ,(1998) , 10.1023/A:1009644315295
R. Goldbach, D. Peters, The biology of tospoviruses. Pathogenesis and host specificity in plant diseases. Histopathological, biochemical, genetic and molecular basis. Vol. 3: Viruses & viroids. pp. 199- 210 ,(1995)
M. A. Matzke, AJM. Matzke, How and Why Do Plants Inactivate Homologous (Trans)genes Plant Physiology. ,vol. 107, pp. 679- 685 ,(1995) , 10.1104/PP.107.3.679
Nigel J. Kilby, H. M. Ottoline Leyser, Ian J. Furner, Promoter methylation and progressive transgene inactivation in Arabidopsis. Plant Molecular Biology. ,vol. 20, pp. 103- 112 ,(1992) , 10.1007/BF00029153
Sheng-Zhi Pang, Fuh-Jyh Jan, Dennis Gonsalves, Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 8261- 8266 ,(1997) , 10.1073/PNAS.94.15.8261
S. P. Kumpatla, W. Teng, W. G. Buchholz, T. C. Hall, Epigenetic transcriptional silencing and 5-azacytidine-mediated reactivation of a complex transgene in rice Plant Physiology. ,vol. 115, pp. 361- 373 ,(1997) , 10.1104/PP.115.2.361
Jan M Kooter, Marjori A Matzke, Peter Meyer, Listening to the silent genes: transgene silencing, gene regulation and pathogen control Trends in Plant Science. ,vol. 4, pp. 340- 347 ,(1999) , 10.1016/S1360-1385(99)01467-3
John J.M.R. Jacobs, Katia Litiere, Victor van Dijk, Gerben J. van Eldik, Marc Van Montagu, Marc Cornelissen, Post-transcriptional beta-1,3-glucanase gene silencing involves increased transcript turnover that is translation-independent Plant Journal. ,vol. 12, pp. 885- 893 ,(1997) , 10.1046/J.1365-313X.1997.12040885.X
Sonia Herrero, Albert K. Culbreath, Alex S. Csinos, Hanu R. Pappu, Rebeca C. Rufty, Margaret E. Daub, Nucleocapsid Gene-Mediated Transgenic Resistance Provides Protection Against Tomato spotted wilt virus Epidemics in the Field. Phytopathology. ,vol. 90, pp. 139- 147 ,(2000) , 10.1094/PHYTO.2000.90.2.139
Sheng- Zhi Pang, Resistance to Heterologous Isolates of Tomato Spotted Wilt Virus in Transgenic Tobacco Expressing Its Nucleocapsid Protein Gene Phytopathology. ,vol. 82, pp. 1223- 1229 ,(1992) , 10.1094/PHYTO-82-1223