Recent developments in latency and recombination of Aujeszky's disease (pseudorabies) virus

摘要: Latency is a characteristic and fascinating part of the biology alphaherpesvirinae, including ADV. Tissue explanation, blot hybridization, in situ hybridization more recently PCR are experimental methods used to demonstrate that latent infections consistently occur ganglionic neurons and, at lower level, tonsillar possibly other cells. In vivo reactivation ADV, resulting shedding virulent has been demonstrated experimentally following administration high doses corticosteriods. To determine influence vaccination with currently gene deleted vaccines on field virus latency load, it essential use quantitative detection methods. We have developed chemiluminescence-based assays specific for gG gE, using these loads tissues pigs vaccinated one several vaccines. Recombination between ADV strains both vitro raised concerns about generation strains. Recent studies mouse model shown concentrations be present same anatomical site recombination take place. This led conclusion ongoing eradication programs, based upon differential serological testing, not likely threatened by vaccine