Development of a sensitive and quantitative method for the identification of two major furan fatty acids in human plasma

摘要: This article focuses on the establishment of an accurate and sensitive quantitation method for analysis furan fatty acids. In particular, sensitivity GC/MS UPLC/ESI/MS/MS was compared identification quantification Different methylation methods were tested with respect to analysis. Special attention needs be paid acids, as acidic catalysts might lead degradation ring. in full-scan mode demonstrated that limit 10 μM. multiple reaction monitoring displayed a higher detection than GC/MS. Moreover, acids charge-reversal derivatization positive two widely used pyridinium salts. Significant oxidation unexpectedly observed using N-(4-aminomethylphenyl) agent. The formed 3-acyl-oxymethyl-1-methylpyridinium iodide derivatized by 2-bromo-1-methylpyridinium 3-carbinol-1-methylpyridinium improved more 2,000-fold nonderivatization negative UPLC/ESI/MS/MS. enabled targeted human plasma. Thus, it is anticipated this protocol could greatly contribute clarification pathological mechanisms related their metabolites.