Influences of DMP on the Fertilization Process and Subsequent Embryogenesis of Abalone (Haliotis diversicolor supertexta) by Gametes Exposure

摘要: Di-methyl phthalate (DMP), a typical endocrine disrupting chemical (EDC), is ubiquitously distributed in aquatic environments; yet studies regarding its impact on gametes and the resulting effects embryogenesis marine gastropods are relatively scarce. In this study, influences of DMP subsequent developmental process abalone (Haliotis diversicolor supertexta, representative benthic gastropod) were assessed. Newborn eggs sperm exposed separately to different concentrations (1, 10 or 100 ppb) for 60 min. At end-point exposure, DMP-treated collected analysis their ultra-structures, ATPase activities total lipid levels, fertilized (embryos) monitor related reproductive parameters (fertilization rate, abnormal development rate hatching success rate). Treatment with did not significantly alter structure content at any doses tested. Hatching failures morphological abnormalities only observed highest dose (100 ppb). However, exposure suppress affect character mitochondria. exhibited dose-dependent decreases fertilization efficiency, morphogenesis hatchability. Relatively obvious toxicological when both DMP. Furthermore, RT-PCR results indicate that treatment changed expression patterns physiologically-regulated genes (cyp3a, 17β-HSD-11 17β-HSD-12) embryogenesis. Taken together, study proofed pre-fertilization eggs, adversely affects