Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways.
作者： Akihiro Yachie , Hideji Nishida , Hiroyuki Tsuchiya , Shinji Miwa , Naotoshi Sugimoto
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摘要: We previously reported that caffeine-assisted chemotherapy improved the treatment of malignant bone and soft tissue tumours such as osteosarcoma. Caffeine affects tumour cells through various pathways, including phosphatase tensin homolog deleted on chromosome 10 (PTEN), AKT, Bcl-2-associated X protein (BAX), caspase-3 p53, has therefore been indicated being useful for tumours. Here, effects caffeine proliferation HOS osteosarcoma were assessed by WST-8 assay, nuclear factor kappa-light-chain-enhancer activated B (NF-κB), mammalian target rapamycin (mTOR) mitogen- kinase (MAPK) pathways western blot analyses. inhibited suppressed NF-κB, mTOR/S6K ERK activities. Our results support those from previous studies relating to use in Osteosarcoma is most common primary children young adults. During last few decades, prognosis patients with
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