Centromeres: Sequences, Structure, and Biology
作者： Cory D. Hirsch , Jiming Jiang
DOI: 10.1007/978-3-7091-1130-7_4
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摘要: Although technological advances have continued to change the speed, cost, and number of plant genomes sequenced (see Flagel Blackman 2012, this volume), parts remain be explored. Even best-sequenced genomes, including Arabidopsis thaliana rice, are missing 7–8% their total genomic information (Kaul et al. 2000; Goff 2002; Yu 2002). One chromosomal region not often in genome projects is centromere. Centromeres almost all higher eukaryotes contain large stretches (up several megabases) tandemly repeated arrays satellite DNA retrotransposons. Such long highly homogenized repetitive sequences cannot readily cloned, sequenced, assembled using currently available cloning sequencing technologies.
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