Standardized kinetic microassay to quantify differential chemosensitivity on the basis of proliferative activity.

摘要: Conventionally in vitro cytotoxicity assays are performed as single-end-point determinations. To compensate for the diversity of growth rates among different cell lines this report we describe a computerized kinetic chemosensitivity assay based on quantification biomass by staining cells with crystal violet. As prerequisite four human breast cancer line (MDA-MB-231, MCF-7, T-47-D and ZR-75-1) were characterized regard to oestrogen progesterone receptor content, modal chromosome number proliferation kinetics depending passages culture. With prolonged time culture ZR-75-1 exposed various concentrations cisplatinum dose-related increase drug effect was observed. Owing correction T/C values initial mass (at when is added) sharp distinction between cytostatic cytocidal effects becomes obvious plots corrected versus incubation. The influence untreated control possible courses theoretical inhibition profiles (reflecting cytostatic, transient cytotoxic or well development resistance) their relationship corresponding curves drugtreated discussed. Chemosensitivity diethylstilbestrol dipropionate, tamoxifen, melphalan, cisplatinum, vinblastine, Adriamycin 5-fluorouracil prove considerations be true MDA-MB-231, practice.