Using Biosensors to Study Free Fatty Acid Receptor Pharmacology and Function

摘要: The free fatty acid (FFA) family of G protein coupled receptors (GPCRs) has generated significant interest for exploiting its members as potential drug targets. However, unravelling the complex pharmacology this proven challenging. In recent years use biosensor technologies capable assessing biological functions in living cells, and real time, greatly enhanced our ability to study GPCR function. These include genetically encoded sensors that change intensity or wavelength light emitted from a bioluminescent fluorescent response stimulus, well non-genetically able measure more global cellular changes, such mass redistribution within cell. This chapter will examine how these can be used GPCRs, particular they are helping uncover FFA receptors.