Microsatellites in Zea - variability, patterns of mutations, and use for evolutionary studies.

摘要: To evaluate the performance of microsatellites or simple sequence repeats (SSRs) for evolutionary studies in Zea, 46 microsatellite loci originally derived from maize were applied to diverse arrays populations that represent all diploid species Zea and 101 inbreds. Although null phenotypes amplification more than two alleles per plant observed at modest rates, no practical obstacle was encountered applying other species. Sequencing revealed complex patterns mutation including frequent indels regions flanking repeats. In one case, variation a locus came region rather repeat motif. Maize show great variability within provide reliable means measure intraspecific variation. Phylogeographic relationships successfully reconstructed with good resolution using genetic distance based on infinite allele model, indicating are useful Zea. Microsatellite principal division between tropical temperate inbred lines, group inbreds these broad germplasm groups manner is largely consistent their known pedigrees.