High Prevalence of Colistin Resistance and mcr-1 Gene in Escherichia coli Isolated from Food Animals in China
作者： Xianhui Huang , Linfeng Yu , Xiaojie Chen , Chanping Zhi , Xu Yao
关键词:
摘要: The objective of this study was to determine the minimal inhibitory concentration colistin for Escherichia coli from food animals and possible underlying resistance mechanisms. During 2007–2014, 4,438 E. isolates animal origins were collected. susceptibility tested by agar dilution method. Mutations in pmrA, pmrB, mgrB presence mcr-1 gene determined PCR DNA sequencing. Complementation experiments carried out evaluate contribution mutations resistance. There a high frequency pigs on farm (24.1%) at slaughter (24.3%) 2013-2014, followed chickens (14.0%) (9.5%). cow lowest (0.9%). MIC distribution showed that most (75.2%) distributed 0.25 mg/L -0.5 mg/L, 4 -8 (16.8%). Compared with recovered during collected 2007-2008 (5.5%) 2010-2011 (12.4%) significantly lower (P<0.05). sequencing complementation failed detect any insertion inactivation or mutation associated However, 91.0% colistin-resistant positive mcr-1. among China urged need minimize potential risks development spread gene.
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