Bioprospecting for Industrial Enzymes: Importance of Integrated Technology Platforms for Successful Biocatalyst Development

摘要: This chapter focuses on the discovery of enzymes from natural environments and optimization by protein engineering directed molecular evolution to develop products that show optimal performance in their target application. Screening large numbers culturable microbes is considered one most successful efficient means finding novel biological compounds such as biocatalysts, this classical route screening will continue be applied with success future. Once best performing candidates have been selected primary secondary screening, genes top are cloned expressed a variety different methods. Cloning expression represent essential steps production industrial today. Industrial applications utilizing biocatalysts often put extreme demands properties due very harsh conditions under processes. Enzyme other than stability-related parameters important functional biocatalyst. There no guarantee enzymatic answer an process can necessarily isolated nature, would demand survival organism depend enzyme relevant for particular Two approaches improving proteins laboratory traditionally taken, rational evolution. Each these two described separately; however, borderline between technologies has become less distinct recent years.