High-performance liquid chromatographic enantioseparation of amino alcohol analogues possessing 1,2,3,4-tetrahydroisoquinoline skeleton on polysaccharide-based chiral stationary phases.

摘要: The stereoisomers of 1,2,3,4-tetrahydroisoquinoline amino alcohol analogues and derivatives thereof were separated in normal-phase mode on chiral stationary phases based preprepared silica coated with cellulose tris-(3,5-dimethylphenyl carbamate), tris-(3-chloro-4-methylphenyl tris-(4-methylbenzoate) or tris-(4-chloro-3-methylphenyl carbamate). On all the investigated columns, retention enantioseparation influenced by nature concentrations mobile phase components additives, also temperature. Experiments performed temperature range 10-50°C. Thermodynamic parameters calculated from plots lnα vs 1/T. these polysaccharide-based both enthalpy-driven separations entropy-controlled enantioseparations observed. latter was advantageous regard to shorter greater selectivity at high sequence elution determined cases.