Nucleic acid isolation and inhibitor removal from complex samples

摘要: The present disclosure provides methods for isolating nucleic acids from a sample, comprising: (a) contacting lysate of the supernatant lysate, or portion with one more first agents (e.g., protein precipitating agents) and second inhibitor removing to generate mixture, (b) separating mixture step into solid phase liquid phase, wherein are primarily in (c) (b). Compositions kits useful such also disclosed. Further disclosed methods, compositions preparing using lytic reagent comprising relatively mild chaotropic phosphates especially complex as soil stool sample.