miR Cluster 143/145 Directly Targets Nrl and Regulates Rod Photoreceptor Development.

摘要: Retinal histogenesis requires coordinated and temporal functioning of factors by which different cell types are generated from multipotent progenitors. Development rod photoreceptors is regulated multiple transcription factors, Nrl one the major involved in their fate specification. Presence or absence at postnatal stages decides generation cone other later retinal cells. This suggests need for expression order to accelerate during development. We found that miR cluster 143/145, comprising miR-143 miR-145, targets imparts a posttranscriptional inhibition Nrl. Expression both miRNAs was differentially development showed least PN1 stage most generated. Downregulation photoreceptor regulators markers upon 143/145 overexpression demonstrated this indeed negatively regulates photoreceptors. Further, we prove positively thus establishing feedback loop regulatory mechanism. may be possible mechanism posttranscriptionally facilitate retina.