Mouse mesenchymal stem cells from bone marrow differentiate into smooth muscle cells by induction of plaque-derived smooth muscle cells.

摘要: Abstract Aim The present study aimed to elucidate the mechanism by which bone marrow mesenchymal stem cells (BMSCs) differentiate into smooth muscle (SMCs) in atherosclerosis. Main methods We isolated mouse BMSCs and incubated them conditioned medium from plaque-derived SMCs (SMC-CM) analyzed growth factors media. were treated with different media harvested at continuous time points for investigating ability toward SMCs. Next, of green fluorescence protein (GFP) mice transplanted apolipoprotein E −/− (apoE ) fed on western type diet 12 weeks. In vivo efficacy was investigated. Key findings After being cultured using SMC-CM, hepatocyte factor (HGF) abundantly secreted time. had increased expression HGF receptor c-met SMC-specific markers while they also displayed SMC characteristic ‘hill valley-like’ appearance an ultra-structure including actin filaments dense bodies. vivo-grafted aggravated atherosclerotic lesions inflammation but ameliorated fibrosis aorta higher levels early not late-stage aorta. They demonstrated greater secretion apoE mice. Furthermore, when blocking antibody, lost Significance local plays important role differentiation homing BMSCs.