Acetylhexosamine compounds enzymically released from Micrococcus lysodeikticus cell walls. III. The structure of DI- and tetra-saccharides released from cell walls by lysozyme and Streptomyces F1 enzyme.
作者： M.R.J. Salton , J.M. Ghuysen
DOI: 10.1016/0006-3002(60)91458-X
关键词:
摘要: Two compounds, both of which have been isolated from cell walls Micrococcus lysodeikticus digested with lysozyme or Streptomyces F1 enzyme, identified as di- and tetra-saccharides. The reducing groups the tetra-saccharides those high-molecular weight, non-dialysable compounds belong to muramic acid. β-glucosidase yields free N-acetylglucosamine N-acetylmuramic acid proposed structure for di-saccharide liberated by is: 6-O-β-N-acetylglucosaminyl-N-acetylmuramic digests appear be identical. tetra-saccharide O-β-N-acetylglucosaminyl-(I→6)-O-β-N-acetylmuraminyl-(I→4)-O-β-N-acetylglucosaminyl- (I→6)-β-N-acetylmuramic acid. Both enzyme degrade tetra-chitobiose, indicating their β(I→4) N-acetyl hexosaminidase activity.
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