A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast.
作者： Robert J. Klebe , June V. Harriss , Z. Dave Sharp , Michael G. Douglas
DOI: 10.1016/0378-1119(83)90238-X
关键词:
摘要: Polyethylene glycol (PEG) can induce genetic transformation in both bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) without cell wall removal. PEG-mediated of E. coli is technically simple yields transformants with an efficiency 10(6)-10(7) transformants/microgram DNA. Detailed analysis the parameters involved reveals basic differences between PEG standard CaCl2 methods for coli. far simpler than existing protoplast comparable efficiency. The new described here may prove to be general utility performing a wide variety organisms.
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J E Suarez, K F Chater, Polyethylene glycol-assisted transfection of Streptomyces protoplasts. Journal of Bacteriology. ,vol. 142, pp. 8- 14 ,(1980) , 10.1128/JB.142.1.8-14.1980
R. P. Mackal, B. Werninghaus, E. A. Evans, THE FORMATION OF λ BACTERIOPHAGE BY λ DNA IN DISRUPTED CELL PREPARATIONS Proceedings of the National Academy of Sciences of the United States of America. ,vol. 51, pp. 1172- 1178 ,(1964) , 10.1073/PNAS.51.6.1172
Fred Sherman, Gerald R. Fink, James B. Hicks, Methods in yeast genetics Cold Spring Harbor Laboratory. ,(1979)
A Weston, M G Brown, H R Perkins, J R Saunders, G O Humphreys, Transformation of Escherichia coli with plasmid deoxyribonucleic acid: calcium-induced binding of deoxyribonucleic acid to whole cells and to isolated membrane fractions. Journal of Bacteriology. ,vol. 145, pp. 780- 787 ,(1981) , 10.1128/JB.145.2.780-787.1981
B J Brown, B C Carlton, Plasmid-mediated transformation in Bacillus megaterium. Journal of Bacteriology. ,vol. 142, pp. 508- 512 ,(1980) , 10.1128/JB.142.2.508-512.1980
Elton T. Young, Robert L. Sinsheimer, Vegetative bacteriophage λ DNA: I. Infectiv in a spheroplast assay☆ Journal of Molecular Biology. ,vol. 30, pp. 147- 164 ,(1967) , 10.1016/0022-2836(67)90250-1
Don B. Clewell, Nature of Col E1 Plasmid Replication in Escherichia coli in the Presence of Chloramphenicol Journal of Bacteriology. ,vol. 110, pp. 667- 676 ,(1972) , 10.1128/JB.110.2.667-676.1972
AKIRA TAKETO, SIGERU KUNO, Sensitivity of Escherichia coli to Viral Nucleic Acid:II. Development of Competence in Escherichia coli CT during Growth in a Hypertonic Medium Journal of Biochemistry. ,vol. 65, pp. 369- 373 ,(1969) , 10.1093/OXFORDJOURNALS.JBCHEM.A129023
AKIRA TAKETO, SIGERU KUNO, Sensitivity of Escherichia coli to Viral Nucleic Acid:I. Effect of Lysozyme, EDTA, Penicillin and Osmotic Shock Treatment Journal of Biochemistry. ,vol. 65, pp. 361- 368 ,(1969) , 10.1093/OXFORDJOURNALS.JBCHEM.A129022
Kimber Hardy, Stephen Stahl, Hans Küpper, Production in B. subtilis of hepatitis B core antigen and of major antigen of foot and mouth disease virus Nature. ,vol. 293, pp. 481- 483 ,(1981) , 10.1038/293481A0