Improving the Sensitivity and Functionality of Mobile Webcam-Based Fluorescence Detectors for Point-of-Care Diagnostics in Global Health.

摘要: Resource-poor countries and regions require effective, low-cost diagnostic devices for accurate identification diagnosis of health conditions. Optical detection technologies used many types biological clinical analysis can play a significant role in addressing this need, but must be sufficiently affordable portable use global settings. Most current optical imaging are sensitive, also expensive difficult to adapt these These challenges mitigated by taking advantage consumer electronics mobile such as webcams, phones, charge-coupled device (CCD) cameras, lasers, LEDs. Low-cost, multi-wavelength fluorescence plate readers have been developed applications including microbial toxins C. Botulinum A neurotoxin, Shiga toxin, S. aureus enterotoxin B (SEB), flow cytometry has detect very low cell concentrations. However, the relatively sensitivities limit their utility. We several approaches improve sensitivity presented here webcam based detectors, (1) image stacking signal-to-noise ratios; (2) lasers enable excitation cytometry; (3) streak capture trajectory single cell, enabling sensors with high noise levels rare events. help overcome some limitations other CCD or phone-based detectors (like sensitivities), provide medical diagnostics resource-poor