IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1.

摘要: Previous studies have established that different cytokines induce distinct patterns of activation in cultured endothelial cells (EC). Treatment EC with either TNF or IL-1 causes transient induction leukocyte adhesion molecule-1 (ELAM-1) and a sustained increase intercellular (ICAM-1) expression. but not also increases class I MHC Ag IFN-gamma, which by itself ICAM-1 does ELAM-1 expression, has been found to act synergistically In our study, we further examined IFN-gamma effects on both beta responses. contrast plus cotreatment, up-regulation molecules is augmented cotreatment beta. expression 24 h whereas cotreated show at most additive increases. TNF-induced such greater number express 4 the presence compared cultures treated alone, although maximal level surface individual increased. Inasmuch as these times represent pre- post-peak time (6 h), respectively, appears accelerate prolong Although similar interactions are seen beta, consistently effect IL-1-induced We next explored possible mechanism(s) synergy between TNF. do cooperatively enhance total protein synthesis. Unexpectedly, combine depress synthesis, may contribute failure positively interact. mRNA 6 h, markedly less cooperative augmentation transcripts. conclude enhances selectively increasing synthesis specific molecules, largely unaffected IFN-gamma.