Identification of a Divergent Lineage Porcine Pestivirus in Nursing Piglets with Congenital Tremors and Reproduction of Disease following Experimental Inoculation

摘要: Congenital tremors is a sporadic disease of neonatal pigs characterized by action-related repetitive myoclonus. A majority outbreaks congenital have been attributed to an unidentified virus. The objectives this project were 1) detect potential pathogen(s) in samples from piglets with and 2) develop infection model reproduce disease. Using next-generation sequencing, divergent lineage pestivirus was detected tremors. virus originally most closely related bat but now more recently published novel porcine provisionally named atypical pestivirus. quantitative real-time PCR the two separate farms, not unaffected same farm. To fulfill second objective, pregnant sows inoculated either serum containing or PBS (control) intravenous intranasal routes simultaneously direct inoculation fetal amniotic vesicles ultrasound-guided surgical technique. Inoculations performed at 45 62 days gestation. All farrowed affected while PBS-inoculated control unaffected. Tremor severity for each piglet scored videos taken 0, 1 2 post-farrowing. remained relatively constant 0 post-farrowing piglets. prevalence pestivirus-inoculated litters ranged 57% (4 out 7 piglets) 100% (10 10 piglets). consistently tissues Samples positive greater than 90% sampled included brainstem (37 41), mesenteric lymph node tracheobronchial whole blood (19 20). Although first description 1922, reported reproduction following experimental Studies investigating mechanism, epidemiology, diagnostic assay development are needed better understand pathophysiology due