Rolling-Circle Amplification Detection of Thrombin Using Surface-Enhanced Raman Spectroscopy with Core-Shell Nanoparticle Probe
作者： Xuemei Li , Linlin Wang , Chunxiang Li
关键词:
摘要: Abstract An ultrasensitive surface-enhanced Raman spectroscopy (SERS) sensor based on rolling-circle amplification (RCA)-increased "hot-spot" was developed for the detection of thrombin. The contains a SERS gold nanoparticle@Raman label@SiO2 core-shell nanoparticle probe in which reporter molecules are sandwiched between core and thin silica shell by layer-by-layer method. Thrombin aptamer sequences were immobilized onto magnetic beads (MBs) through hybridization with their complementary strand. In presence thrombin, sequence released; this allowed remaining single-stranded DNA (ssDNA) to act as primer initiate situ RCA reaction produce long ssDNAs. Then, large number probes attached ssDNA templates, causing thousands be involved each biomolecular recognition event. This method achieved thrombin range from 1.0×10(-12) 1.0×10(-8) M limit 4.2×10(-13) M, showed good performance real serum samples.
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